Concept of a non-invasive, simple method for monitoring the decellularization coefficient using vision-based techniques
DOI:
https://doi.org/10.34767/SIMIS.2025.03.01Keywords:
biomaterials; decellularization; quality control; optical methods; extracellular matrix (ECM)Abstract
The aim of this study was to develop a simple, non-invasive method for monitoring the progress of tissue decellularization using measurements of changes in visible-light transmission. The proposed approach is based on the empirically observed increase in optical transparency that accompanies the removal of cellular components, which corresponds to a reduction in the effective attenuation coefficient μ. Three design variants of the measurement system were presented—a single-detector prototype, a laser-based configuration, and a CMOScamera module—each enabling detection of changes in the transmission coefficient T during the process. Under stable measurement conditions, the method allows real-time assessment of the degree of decellularization without damaging the material and can serve as a supplement to classical laboratory techniques. The presented concept provides a foundation for further experimental validation and optimization of the measurement system for use in laboratory and production settings.
References
Ashby MF, Ferreira PJ, Schodek DL Material Classes, Structure, and Properties. Nanomaterials, Nanotechnologies and Design. 2009; :87–146.
From Definitions to Regulations of Medical devices and Materials - BIOMATDB [Internet]. [dostęp01.11.2025]. Dostępne na: https://www.biomatdb.eu/2023/08/05/fromdefinitions-to-regulations-of-medical-devicesand materials/
Wagner WR., Sakiyama-Elbert SE., Zhang Guigen, Yaszemski MJ.: Biomaterials science: an introduction to materials in medicine. Academic Press, an imprint of Elsevier; 2020. 1616 s.
Badylak SF: Xenogeneic extracellular matrix as a scaffold for tissue reconstruction. Transpl Immunol. 2004; 12(3–4):367–377.
Faulk DM, Wildemann JD, Badylak SF: Decellularization and Cell Seeding of Whole Liver Biologic Scaffolds Composed of Extracellular Matrix. J Clin Exp Hepatol. 2015; 5(1):69–80.
Gilbert TW, Sellaro TL, Badylak SF: Decellularization of tissues and organs. Biomaterials. 2006; 27(19):3675–3683.
Gilbert TW, Sellaro TL, Badylak SF: Decellularization of tissues and organs.Biomaterials. 2006; 27(19):3675–3683.
Faulk DM, Wildemann JD, Badylak SF: Decellularization and cell seeding of whole liver biologic scaffolds composed of extracellular matrix. J Clin Exp Hepatol. 2015; 5(1):69–80.
Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, i in.: Perfusiondecellularized matrix: using nature’s platform to engineer a bioartificial heart. Nature Medicine 2008 14:2. 2008; 14(2):213–221.
Moloney MP, Govan J, Loudon A, Mukhina M, Gun’ko YK: Preparation of chiral quantum dots. Nature Protocols 2015 10:4. 2015; 10(4):558–573.
Jacques SL: Optical properties of biological tissues: a review. Phys Med Biol. 2013; 58(11):R37.
Bhatt M, Ayyalasomayajula KR, Yalavarthy PK: Generalized Beer–Lambert model for near-infrared light propagation in thick biological tissues. J Biomed Opt. 2016; 21(7):076012.
LAMBERT JHEINRICH: LAMBERTS PHOTOMETRIE : (photometria, sive de mensura et gradibusluminis, colorum etumbrae)... (1760) [Internet]. WENTWORTH PRESS; 2018.
Huang J, Ou Z: Refractive Index Engineering: Insights from Biological Systems for Advanced Optical Design. 2025; .
Solouma N, Hamdy O: Ex Vivo Optical Properties Estimation for Reliable Tissue Characterization. Photonics 2023, Vol 10, Page 891. 2023; 10(8):891.
Turbidity sensor SKU SEN0189 [Internet]. [dostęp24.11.2025]. Dostępnena: https://wiki.dfrobot.com/Turbidity_sensor_SK U__SEN0189
opencv-python · PyPI [Internet]. [dostęp24.11.2025]. Dostępne na: https://pypi.org/project/opencv-python/
Raspberry Pi Documentation [Internet]. [dostęp24.11.2025]. Dostępne na: https://www.raspberrypi.com/documentation/
Pereira RHA, Prado AR, Caro LFC Del, Zanardo TÉC, Alencar AP, Nogueira BV: A non-linear mathematical model using optical sensor to predict heart decellularization efficacy. Scientific Reports 2019 9:1. 2019; 9(1):12211-.
Mora-Navarro C, Garcia ME, Sarker P, Ozpinar EW, Enders JR, Khan S, i in.: Monitoring decellularization via absorbance spectroscopy during the derivation of extracellular matrix scaffolds. Biomed Mater. 2021; 17(1):10.1088/1748-605X/ac361f.
Salgado García MG, Díaz NF, García López G, Álvarez Maya I, Hernández Jimenez C, Roman Maldonado Y, i in.: Evaluation methods for decellularized tissues: A focus on human amniotic membrane. J Biosci Bioeng. 2025; 139(2):85–94.
He C, He H, Chang J, Chen B, Ma H, Booth MJ: Polarisation optics for biomedical and clinical applications: a review. 2021.
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